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 LiudmilaD

link 11.02.2012 20:17 
Subject: diluent fold gen.
Counting: count according to colony count principle and multiply with diluent fold to calculate bacterial colony count of each piece of product (cfu/pc).

 Ivanog

link 11.02.2012 21:30 
А можно параграф целиком? Не очень понятно о чем речь.

 LiudmilaD

link 11.02.2012 21:34 
если я правильно перевела, речь идет о тестировании на бактериальное загрязнение (Initial Contamination Bacteria Test),
метод тестирования
Test Method: (plate-count method)
1. Eluent: 0.1% peptone, 0.05% Tween-80, 0.9% NaCl solution.
2. Sample treatment: Decompose the product by sterile operation and move them into eluent, vibrate in vortex mixer for 2 minutes for use.
3. Aerobe culturing: product eluent 2ml inoculated to 2 plates respectively, 1 ml for each plate; each plate is injected with 20ml TSA, cultivating for 48-72h under 30-35℃.
4. Mold culturing: product eluent 2ml inoculated to 2 plates respectively, 1 ml for each plate; each plate is injected with 20ml SDA, cultivating for 5-7 days under 20-25℃.
5. Counting: count according to colony count principle and multiply with diluent fold to calculate bacterial colony count of each piece of product (cfu/pc).

 Rengo

link 11.02.2012 21:38 
умножают на соотвествующее разведение

 OlgaAvdeeva

link 12.02.2012 6:53 
+1 (или ...на коэффициент разведения)

 

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